In my opinion most of the antivirus/antispyware systems are not that helpful in stopping/finding all spyware on the computer.
When I go to a someone's house to fix their computer due to spyware. I do run AVG antispyware to help find any of the obvious adware/spyware on the computer. But since they use signature based detection it can still miss a lot.
So I then grab some tools from sysinternals.com such as autoruns,process explorer, rootkit realver. Boot the computer in safe mode and remove the bad startup/services/login processes. Manually review over the windows directory and system32 directory by date/time and filename.
Also grab unlocker from http://ccollomb.free.fr/unlocker/ which will allow you to remove files that are in use.
If you need to get a decent free one I tend to suggest AVG Antispyware it changes all the time I used to like Spysweeper as a good free one but feels like it has become bloated and slow.
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